one-way anova and kruskal–wallis/dunn’s multiple comparison tests using graphpad prism v10  (GraphPad Software Inc)

Journal: bioRxiv

Article Title: Dual role of Arabidopsis SRS2 helicase in meiotic recombination

doi: 10.1101/2025.02.26.640294

Figure Lengend Snippet: (A) Representative pictures of 2-week-old seedlings grown without (left), or with MMC (middle: 30 µM; right: 40 µM). (B) Mean number of true leaves per seedling counted by direct visual inspection. Data are represented as mean ± SEM of 3 independent experiments. P values were calculated using nonparametric statistical analysis (Kruskal–Wallis test). ** p-value < 0.01. rad51 is RAD51-GFP transgenic line described in (C) Mean number of true leaves per seedling of several helicase mutants and double mutant lines counted under a binocular microscope. Data are represented as mean ± SEM. Statistical analysis was performed using Kruskal-Wallis test; * p-value < 0.05. (D) Quantification of the number of spontaneous HR events (blue sectors) in somatic cells using IU-GUS assay, with n indicating the number of seedlings analysed. Each mutant was compared to wild-type (SRS2+/+, grey boxes) sister plants. Data are represented as mean ± SEM. P values were calculated using nonparametric statistical analysis (Kruskal–Wallis test).

Article Snippet: Statistical analysis of the number of RAD51 foci per nucleus was performed using Kruskal Wallis test (GraphPad Prism v10.4.0 software).

Techniques: Transgenic Assay, Mutagenesis, Microscopy, GUS Gene Assay

Journal: bioRxiv

Article Title: Dual role of Arabidopsis SRS2 helicase in meiotic recombination

doi: 10.1101/2025.02.26.640294

Figure Lengend Snippet: (A-B) Immunolocalization of RAD51 in root tip nuclei of 5-days-old seedlings untreated (A) or treated with 30 µM MMC for 6h (B) . Experiments were performed on srs2-1 and srs2-3 mutant lines. DNA is stained with DAPI (blue) and RAD51 foci (detected using an antibody against RAD51) are colored in green. Images are collapsed Z-stack projections of 3D image stacks. Scale bar: 5 µm. (C) Quantification of RAD51 foci in root tip nuclei of WT, srs2-1, and srs2-3 mutant lines before and after MMC treatment. Data are shown as mean ± SD, with n indicating the number of cells analyzed. More than 300 nuclei from at least 3 seedlings were analyzed per genotype. P values were calculated using nonparametric statistical analysis (Kruskal–Wallis test); * p-value < 0.05; **** p-value < 0.0001. (D) Percentage of cells with 0, 1-2, 3-10, 11-20, 21-50, or > 50 RAD51 foci for each genotype, before and after MMC treatment. Cells analyzed are the same as in (C) .

Article Snippet: Statistical analysis of the number of RAD51 foci per nucleus was performed using Kruskal Wallis test (GraphPad Prism v10.4.0 software).

Techniques: Mutagenesis, Staining

Journal: bioRxiv

Article Title: Dual role of Arabidopsis SRS2 helicase in meiotic recombination

doi: 10.1101/2025.02.26.640294

Figure Lengend Snippet: (A) Plant fertility was measured by counting the number of seeds per silique, with n indicating the number of siliques analyzed. Each dot represents one silique. Mean is represented as a red line. Statistical analysis was performed using nonparametric Kruskal-Wallis test; ns : not significant (p-value > 0.05). (B) DAPI-stained male meiotic nuclei. Normal meiotic progression occurs in srs2 mutants, with complete synapsis at pachytene, five intact bivalents at metaphase I, two pools of five chromosomes at Anaphase I, and tetrads with four balanced meiotic products. Scale bar: 10 µm. (C) Co-immunolocalization of RAD51 (magenta) and the chromosome axis protein ASY1 (green) on leptotene/zygotene meiotic chromosome spreads. Scale bar: 5 µm. (D) Co-immunolocalization of RAD51 (magenta) and ZYP1 (green) on zygotene/pachytene meiotic chromosome spreads. Scale bar: 5 µm. (E) Chiasma number per cell at metaphase I stage, with n indicating the number of cells analyzed. The number of chiasmata per cell was estimated based on the shape of the bivalents. Statistical analysis was performed using parametric unpaired t-test; ns : not significant (p-value > 0.05).

Article Snippet: Statistical analysis of the number of RAD51 foci per nucleus was performed using Kruskal Wallis test (GraphPad Prism v10.4.0 software).

Techniques: Staining

Journal: bioRxiv

Article Title: Dual role of Arabidopsis SRS2 helicase in meiotic recombination

doi: 10.1101/2025.02.26.640294

Figure Lengend Snippet: (A) FTL crossover frequency (I1bc interval) in wild-type (grey), srs2-1 (blue) and srs2-3 (green). CO frequency within I1bc interval is presented as the genetic distance. Each mutant is compared to wild-type (WT) sister plants (SRS2 +/+, in grey). Each dot represents one plant. Means are indicated by red horizontal lines. (B) Crossover Interference within I1bc interval in WT, srs2-1 and srs2-3 mutants. Each mutant is compared to wild-type sister plants that are SRS2 +/+ (in grey). Each dot represents one plant. Means are indicated by red horizontal lines. Statistical analysis was performed using Z-test. ** p-value < 0.01; *** p-value < 0.001. (C) Representative images of MLH1 (magenta) immunolocalization on diakinesis-staged male meiocytes in wild-type and srs2 mutants. Scale bar: 10 µm. (D) Number of MLH1 foci per cell, with n indicating the number of cells analyzed. Each dot represents the total number of MLH1 foci in an individual cell. Mean is presented as a red bar. Statistical analysis was performed with unpaired Kruskal-Wallis test; * p-value < 0.05. (E) Representative images of MLH1/HEI10 co-immunolocalization on diakinesis-staged male meiocytes in wild-type and srs2 mutants. Scale bar: 10 µm. (F) Number of MLH1/HEI10 co-foci per cell, with n indicating the number of cells analyzed. Each dot represents the total number of MLH1/HEI10 co-foci in an individual cell. Mean is presented as a red bar. Statistical analysis was performed with unpaired Kruskal-Wallis test; * p-value < 0.05.

Article Snippet: Statistical analysis of the number of RAD51 foci per nucleus was performed using Kruskal Wallis test (GraphPad Prism v10.4.0 software).

Techniques: Mutagenesis